NGS LIBRARY PREPARATION: A TECHNOLOGICAL BREAKTHROUGH IN THE HEALTHCARE SECTOR
The healthcare sector is witnessing several technological disruptions that are reshaping it. Applications developed with the help of artificial intelligence (AI) are flooding the healthcare sector. One of the main contributions of AI has been the Next Generation Sequencing (NGS). NGS library preparation is all about preparing nucleic acid targets, using a coordinated series of enzymatic reactions to produce a random collection of specifically sized DNA fragments, for high throughput sequencing. The simultaneous sequencing of millions of DNA molecules have been made possible by NGS.
In the last ten years, NGS has seen massive growth and there is a prominent sign of it growing further in the coming years. Sequencing is being adopted as a standard form of technology in different applications. A leading market research company has come up with a research report on the global NGS library preparation market. The report states that the global NGS library preparation market is slated to reach approximately USD 1.33 Bn by 2023, expanding at a compound annual growth rate (CAGR) of 11.3% during 2018-2023. The decreasing costs of NGS library preparation solutions have contributed to the wide application and affordability of this technology.
Application of NGS
Many applications of the NGS technology has been observed in the recent times. Researchers are globally adopting NGS technology all across the world. Here are some applications of the NGS technology:
DNA-sequencing, RNA sequencing, epigenome sequencing is being used by researchers for measuring genetic variations in an organism with an existing reference genome. DNA sequencing NGS technology uses whole genome, whole exome and targeted sequencing. NGS technology is being used to track genetic and structural variations.
De novo sequencing:
De novo sequencing is being used by researchers increasingly for building new genome from unknown organisms. This can be done with the help of an ‘assembler’ to put fragmented DNA threads together.
This technology along with chromatin immunoprecipitation is being used by researchers for genetic studies and regulatory mechanisms used by the genome, followed by sequencing. In CPG dinucleotides, the presence of methylation is being detected using the bisulfite sequencing method.
DNA and RNA sequencing:
This is being used by researchers to analyse transcriptome results. RNA splicings, mutations, gene fusion and differential gene expression are used by researchers to get the transcriptome results.
Apart from these, NGS technology is also being used by microbial ecology scientists to gather genetic materials from environmental samples.
There are several challenges that the NGS library preparation market is facing currently. Academic institutions are not increasing their capital investment in NGS and this is considerably hampering research and development scope. This is also making the NGS library preparation market more and more dependent on the grants given by governments. With such financial deficit, it becomes difficult to implement more developmental plans in this sector. With less research happening there is no standardization and lack of accuracy. Lack of data storage facility is also posing a great challenge to the NGS preparation market.